High performance liquid chromatography (HPLC) is a widely used technique, especially for analysis of polar, thermo-labile and non-volatile substances.

The training covers following topics:
- LC fundamentals
- Hands-on training
- Maintenance and troubleshooting

Duration: 3.5 working days / Maximum number of participants: 4

Detailed time schedule:
Day 1 – a.m. – Theoretical session HPLC
HPLC Fundamentals:
• Theoretical aspects of HPLC
• Selection of mobile phase
• Selection of stationary phase
• Optimisation of working conditions, separation efficiency

Day 1 – p.m. – Practical session HPLC
Experiments and demonstrations related to:
• Setup of optimum working conditions
• Separation efficiency and column parameters

Day 2 – a.m. – Theoretical session HPLC
HPLC Fundamentals:
• Pumps
• Injection methods
• Detectors (UV, DAD UV, FLD)
• Qualitative analysis

Day 2 – p.m. – Practical session HPLC
Experiments and demonstrations related to:
• DAD UV, FLD and other detectors
• Qualitative analysis, spectral library

Day 3 – a.m. – Theoretical session HPLC
Applied HPLC:
• Method development
• Main applications
• Quantitative analysis

Day 3 – p.m. – Practical session HPLC
Experiments and demonstrations related to:
• Method development
• Quantitative analysis

Day 4 – a.m. – Practical session HPLC
Experiments and demonstrations related to:
• Maintenance and troubleshooting