Mass spectrometry (MS) is a key technique in any advanced analytical laboratory allowing for sensitive and selective determinations of wide physico-chemical properties range compounds. The obtained mass spectra can be used for identification of unknown substances.
The training covers following topics:
- Basic principles of the most frequently used ionisation techniques
- Interpretation of mass spectra and quantitative analysis
- Hands-on training
Duration: 4.5 working days / Maximum number of participants: 4
Detailed time schedule:
Day 1 – a.m. – Theoretical session Mass Spectrometry
Fundamental aspects of mass spectrometry:
• Theoretical aspects of mass spectrometry
• Different type of analysers (quadrupole, ion trap, triple quadrupole, TOF, Q-TOF)
• Modes of operation (full scan, SIM)
Day 1 – p.m. – Practical session Mass Spectrometry
Experiments and demonstrations related to:
• Modes of operation (full scan, SIM)
Day 2 – a.m. – Theoretical session Mass Spectrometry
Mass spectrometric detectors coupled to GC:
• Different ionisation techniques (EI, CI, etc.)
Day 2 – p.m. – Practical session Mass Spectrometry
Experiments and demonstrations related to:
• GC-MS
Day 3 – a.m. – Theoretical session Mass Spectrometry
Mass spectrometric detectors coupled to LC:
• Different ionisation techniques (APCI, ESI, etc.)
• Interfaces
Day 3 – p.m. – Practical session Mass Spectrometry
Experiments and demonstrations related to:
• LC-MS
Day 4 – a.m. – Theoretical session Mass Spectrometry
Qualitative and quantitative analysis using MSD:
• Molecular ions and isotopic clusters
• Basic principles of fragmentation
• Spectra interpretation
• Principles for quantification
Day 4 – p.m. – Practical session Mass Spectrometry
Experiments and demonstrations related to:
• Interpretation of mass spectra
• Qualitative analysis
• Spectral libraries and their use
• Quantitative GC-MS using EI and IS calibration
Day 5 – a.m. – Theoretical session Mass Spectrometry
Demonstrations related to maintenance of MSD:
• Source cleaning
• Troubleshooting